Drage Rode,

ne mogu ne podijeliti ovaj clanak objavljen ovaj mjesec u Fertility and Sterility, evo linka

ukratko:
- 41 zena u studiji, 11 dobile donor eggs
- radili biopsiju na polar body 1 (prije fertilizacije), polar body 2 (nakon fertilizacije) i blastici, metoda se zove CGH (comparative genomic hybridization) i gleda da li ima aneuplodija (extra ili manjak kromozoma) u stanici
- 39% jajnih stanica je bilo normalno (!!!! vecina nasih jajnih stanica ne moze napraviti bebu!)
- kad su se normalne jajne stanice oplodile, 88% is je bilo normalno
- 95% normalnih embrija je doslo do blastice
- 6 od 41 zene nije imalo nijedan normalni embrio i nije imalo transfer
- od 35 zena koje su imale transfer, transferirane su jedna ili dvije blastice
- postotak uspjeha 82% (postotak implantacije 74%)
- 6 od 35 su imale blizance (15% nasuprot 40% u normalnom IVF-u).

kopiram abstract:

OBJECTIVE: To assess the karyotypic relationship between prefertilized/postfertilized oocytes and embryos using comparative genomic hybridization (CGH) on polar body-1 (PB-1), PB2, and blastomere biopsies and to evaluate IVF outcomes after transfer of blastocysts derived from euploid oocytes. DESIGN: Prospective cohort. SETTING: Medical center. PATIENT(S): Phase1: Fourteen oocyte donors (23-29 years). Phase 2: Forty-one healthy embryo recipients aged 29-43 years free of endometrial implantation dysfunction. In 30 cases own eggs were used. Eleven women used donated oocytes. INTERVENTION(S): Phase 1: PB-1 biopsies followed intracytoplasmic sperm injection (ICSI), PB-2, and day 3 blastomere biopsies. Phase 2: PB-1 biopsy followed by ICSI using normal sperm and the subsequent embryo transfer of </=2 blastocysts derived from euploid oocytes. Comparative genomic hybridization on all DNA derived from phase 1 and 2 biopsies. MAIN OUTCOME MEASURE(S): Pregnancy and implantation rate. RESULTS: Phase 1: 39% of oocytes and 88% of zygotes were euploid; >95% progressed to blastocysts. Mosaicism as evidenced by euploid oocytes developing into aneuploid zygotes or embryos occurred in 13% of concepti. Phase 2: Six of 30 women using own eggs, who failed to produce euploid oocytes, were cancelled. Thirty-five women underwent embryo transfers with </=2 (mean, 1.3 +/- 0.7) blastocysts derived from euploid oocytes. The ongoing pregnancy/implantation rates per embryo transfer were 74% and 82%, respectively. CONCLUSION(S): Transferring euploid embryos markedly improved IVF outcome. These findings, if corroborated, could initiate a paradigm shift in assisted reproductive technology (ART).

Idem se dokopati cijelog clanka pa saljem koga zanima!